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KMID : 1094720100150061065
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 6 p.1065 ~ p.1070
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Asiaticoside production from centella (Centella asiatica L. Urban) cell culture
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Loc Nguyen Hoang
An Nguyen Thi Tam
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Abstract
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Petiole explants of centella plants (Centella asiatica L. Urban) were cultured on Murashige and Skoog (MS) solid medium containing 20 g/L sucrose, supplemented with 1.0 mg/L benzylaminopurine and 1.0 mg/L naphthaleneacetic acid for callus production. To establish a cell suspension culture, 2 g of fresh callus was cultured in 50 mL of the same medium but without solid agent at a 100 rpm agitation speed. Every 2 g of culture was subcultured in fresh MS liquid medium for maintenance. After 24 days of culture at a 120 rpm agitation speed, the centella cell biomass reached a maximum of 9.03 g/50 mL on the same MS medium with 30 g/L sucrose and a 3 g inoculum size. A high performance liquid chromatography analysis showed that asiaticoside content in 24-day old suspension cultured cells (45.35 mg/g dry weight) was significantly higher (4.5 fold) than that of in planta leaves (10.55 mg/g dry weight).
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KEYWORD
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callus, cell suspension, asiaticoside, HPLC, Centella asiatica
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